Innovation – Open view page
General information
| Innovation ID | 286 |
| Version ID | 215 |
| Innovation Title | Loop-mediated Isothermal Amplification (LAMP) assay protocol for field detection of Ralstonia solanacearum spp |
| Innovation description | Seed potato certification standards have zero tolerance for bacterial wilt (caused by Ralstonia solanacearum) leading to rejection of entire potato seed fields. Current seed potato certification process involves costly and time consuming diagnosis protocols. This molecular method is a validated cost-effective LAMP assay for the detection of Ralstonia from potato stem, leaf, tuber and soil also in the field. |
| Reporting Staff | Kalpana Sharma (CIP) |
| Year (Reporting) | 2019 |
| Reporting status | Approved |
| Innovation Type | Research and Communication Methodologies and Tools |
Stage reached
| Stage of Innovation | Stage 2: successful piloting |
| Year (Stage) | 2019 |
| Stage Description | On-site diagnosis of bacterial wilt disease using the validated LAMP assays has been demonstrated to Rwandan Agricultural Board (RAB) researchers/lab technicians and plant diagnostic experts of Crop Nutrition Laboratory Services (CROPNUTS), a private agricultural laboratory and agronomy services company based in Nairobi Kenya. |
| Has a lead organization | yes |
| Lead Organization | International Potato Center |
| Top 5 contributors | Kenya Plant Health Inspectorate Service |
Contributions and mapping
| All partners | International Potato Center-CGIAR Center/Program (Center); Kenya Plant Health Inspectorate Service-Government; Rwanda Agriculture and Animal Resources Development Board-National Agricultural Research System (NARS) |
| Main CGIAR portfolio | CGIAR Research Program on Roots, Tubers and Bananas |
| Flagship project | FP2-Adapted productive varieties and quality seed of RTB crops |
| Cluster | PO2.4-Improving Livelihoods of Potato Farmers in Africa by Tackling Deteriorated Seed Quality through an Integrated Approach |
| Other CGIAR portfolios-Flagships-Clusters |
Scope
| Geographic scope | Regional |
| Regions | Eastern Africa |
| Countries | Kenya,Rwanda |
Targeted outcomes
| Main Sub-IDO | Reduced production risk |
| Other Sub-IDO | Enhanced institutional capacity of partner research organizations |
| Other Sub-IDO |
Evidences
| Evidences | "Steps in Field-deployable LAMP assay.""Abdulwahab Abdurahman. (31/7/2019). Validation of Loop-Mediated Isothermal Amplification Assays for Field Detection of Palstonia Solanacearum Species Complex Strains.""Kalpana Sharma. (1/8/2019). Fast Tracking of Certified Seed Potato Through the Use of Field Deployable LAMP Assay.""Kalpana Sharma. (9/10/2019). Loop-mediated Isothermal Amplification (LAMP) assay protocol for field detection of Ralstonia solanacearum spp. Peru: International Potato Center (CIP)." |
Linked Elements
| Milestones |
| Outcome Impact Case |
| Policy |