Innovation – Open view page
General information
| Innovation ID | 499 |
| Version ID | 387 |
| Innovation Title | Protocol for sweetpotato leaf collection using locally available material for accurate varietal identification based on DNA fingerprinting in Malawi |
| Innovation description | Sweetpotato leaf collection for varietal identification is key for accurately understand varietal impact at household level. However, logistics to collect and conserve leaves can be complicated, specially in sub-saharan countries with poor road infrastructure and few places to keep cold the material during survey development. To overcome this problem, we developed a protocol to use locally available products together with high quality standard for managing plant material for DNA extraction. |
| Reporting Staff | Julius Juma Okello (CIP), Willy Pradel Caceres (CIP), Guy Gaston Hareau (CIP), Felistus Chipungu (CIP), Marcel Gatto (CIP), Noelle Anglin (CIP) |
| Year (Reporting) | 2020 |
| Reporting status | Approved |
| Innovation Type | Research and Communication Methodologies and Tools |
Stage reached
| Stage of Innovation | Stage 3: available/ ready for uptake |
| Year (Stage) | 2020 |
| Stage Description | Currently, the protocol was validated at field level during a nation-wide survey in Malawi in 2019. Six leaf collectors were in charge to apply the protocol after two-week training. Later, the material was sent to a lab in Australia that verified the high quality DNA from the dried leaves. |
| Has a lead organization | yes |
| Lead Organization | International Potato Center |
| Top 5 contributors | No contributors |
Contributions and mapping
| All partners | International Potato Center-CGIAR Center/Program (Center); Ministry of Agriculture Irrigation and Water Development, Department of Agricultural Research Services-Government; Diversity Arrays Technology Pty Ltd-Private Sector; Ministry of Agriculture Irrigation and Water Development, Department of Agricultural Research Services, Bvumbwe Agricultural Research Station-Advanced Research Institutions |
| Main CRP | CGIAR Research Program on Roots, Tubers and Bananas |
| Flagship project | FP5-Improved livelihoods at scale |
| Cluster | CC5.1-Foresight and impact assessment |
| Other CRPs-Flagships-Clusters |
Scope
| Geographic scope | National |
| Regions | |
| Countries | Malawi |
Targeted outcomes
| Main Sub-IDO | More efficient use of inputs |
| Other Sub-IDO | |
| Other Sub-IDO |
Evidences
| Evidences | Pradel, W. 2021. Protocol for sweetpotato leaf tissue collection. Blog, published March 24, 2021 [https://hdl.handle.net/20.500.11766.1/1f0f1a] "Willy Pradel Caceres, Felistus Chipungu, Marcel Gatto, Julius Juma Okello, Noelle Anglin, Rocio Isabelle Alagon Talaverano, Guy Gaston Hareau, Clementina Banda. (24/6/2020). Protocol to follow by enumerators to confirm genetic integrity of putative sweetpotato field plants. Peru: International Potato Center (CIP).""Willy Pradel Caceres, Noelle Anglin, Felistus Chipungu, Guy Gaston Hareau, Julius Juma Okello, Marcel Gatto, Clementina Banda. (17/12/2020). Adoption study of sweetpotato varieties in Malawi: DNA Fingerprinting Activity. Peru: International Potato Center (CIP)." |
Linked Elements
| Milestones | 6695-154-30036 - At least three ex-post impact assessment studies published. |
| Outcome Impact Case |
| Policy |