ABOUT US

PAK-US Cotton Productivity Enhancement Project (2012-17) is being executed in collaboration with ICARDA. It is launching several breeding programs to evolve virus free varieties of cotton in Pakistan.ICARDA has divided the project into 13 different smaller projects and allocated them to some of the best public research departments such as NIBGE, CCRI, NUST, CMBE, Punjab Universty etc. USDA is providing technical support to Pakistani scientists on cultivation of US germplasm in Pakistan.

For more information please visit full project website

Period of Implementation

Sep 14, 2012 - Jun 30, 2017
Total Budget

USD 713,000.00

OUR IMPACT

Goals

To develop CLCV-resistant cotton variety, in the wake of success in evolving virus-free cotton line from US germplasm

Objectives

To develop CLCUV resistant germplasm for Pakistan using traditional breeding approaches To manage cotton disease IPM techniques by adopting farmer's field school To develop gene constructs and genetically engineered cotton germplasm resources To establish cotton virology and whitefly haplotyping facilitation unit

Problems and Needs Analysis

In past few decades cotton leaf curl disease (CLCuD) caused by Cotton leaf Curl Virus (CLCuV) has caused notable decrease in the cotton yield which resulted in major losses to the Pakistan economy. Pakistan being fourth largest cotton producing country of the world, Its share in total world cotton production was 9.47% in 2004/2005. All efforts to increase cotton production in Pakistan did not become fruitful because of large losses due to CLCuD. It has seriously hampered the cultivation and production of cotton crop in Pakistan over US$ 5 billion losses to the Pakistan economy during the mid to late 1990s. This project will help to screen the resistant and susceptible varieties against CLCuD and to generate resistant cotton varieties before the possible outbreak of disease.

Intervention Strategy(ies)

CLCV is the major reason for the decline in Pakistan’s cotton and textile exports. During the last fiscal year (July-June 2011-12), Pakistan’s textile sector export declined to US$ 12.35 billion from US$ 13.78 billion in the same period last year. Cotton leaf curl disease (CLCuD) complex is a debilitating disease of cotton that results in leaf curling, development of leaf-like enations on the undersides of leaves, overall stunting of the plant, and reduced yield and quality. The disease is caused by one or more whitefly-transmitted geminiviruses. The development of disease resistant seeds and germplasm has become essential.

Impact Pathway

The project will screen the resistant and susceptible varieties against CLCuD and will grow resistant cotton varieties. In order to achieve its targets, intensive lab research will be done to develop gene constructs and genetically engineered cotton germplasm resources which will reduce cotton production risk (1.1.2) in the country and increase value capture by farmers (1.3.3) at ground level. Research on CLCUV resistant Germplasm will enhance genetic gain (1.4.3) and is expected to increase resilience of poor & vulnerable communities (3.3.1) against CLCU viral shocks. Moreover, cotton virology and whitefly haplotyping facilitation unit will increase institutional capacity of partner organization (D 1.1) to develop gene constructs, mapping and DNA sequencing and ultimately it will improve capacity for innovation in partner research organization (D 1.3).

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WHERE WE WORK

RESOURCES

Amplicon-based RNA interference targeting V2 gene of cotton leaf curl kokhran virus- burewala strain can provide resistance in transgenic cotton plants

Author(s): Sarfraz Kiani | Afshan Butt | Abdul Qayyum Rao | Aftab Ahmad | Idrees Ahmad Nasir | Tayyab Husnain | Shahid Mansoor | Imran Amin | Shaheen Aftab | Muhammad Zubair | Muhammad Nouman Tahir | Sohail Akhtar | Jodi Scheffler | Brian Scheffler | Faheem Akram

Date: 2016-10-15 | Type: Journal Article

A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters

Author(s): Shaista Javaid | Shaista Javaid | Shaista Javaid | Imran Amin | Georg Jander | Zahid Mukhtar | Nasir A Saeed | Shahid Mansoor

Date: 2016-10-06 | Type: Journal Article

Study of Secondary Endosymbionts of Whiteflies Collected from Cotton Growing Districts of Punjab, Pakistan

Author(s): Muhammad Yousaf Ali | Muhammad Yousaf Ali | Imran Amin | Sohail Akhtar | Shahid Mansoor

Date: 2016-08-01 | Type: Journal Article

Transient expression of synthetic coat protein gene of Cotton leaf curl Burewala virus in tobacco (Nicotiana benthamiana)

Author(s): Imran Amin | Shahid Mansoor | Muhammad Saeed | Ghulam Rasool

Date: 2016-07-29 | Type: Journal Article

RNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly

Author(s): Hassan Jamil | Muhammad Shafiq | Imran Amin | Jodi Scheffler | Brian Scheffler | Shahid Mansoor | Amir Raza

Date: 2016-04-19 | Type: Journal Article

First Report of Chilli leaf curl virus and Associated Alpha- and Beta-satellite DNAs Infecting Nettle Weed (Urtica dioica) in Pakistan

Author(s): Javaid Iqbal | W. Hussain | M. Zia-Ur-Rehman | U. Hameed | M. S. Haider

Date: 2016-04-01 | Type: Journal Article

Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host

Author(s): Iftikhar Ali | Khalid Pervaiz Akhtar | Fazal Akbar | Ishtiaq Hassan | Imran Amin | Muhammad Saeed | Shahid Mansoor

Date: 2016-02-01 | Type: Journal Article

Virus-Induced Gene Silencing in Cultivated Cotton (Gossypium spp.) Using Tobacco Rattle Virus.

Author(s): Muhammad Shafiq | Shahid Mansoor | Rob W. Briddon | Brian Scheffler | Jodi Scheffler | Imran Amin | Roma Mustafa

Date: 2015-11-24 | Type: Journal Article

Genetic Diversity Analysis for Earliness, Fiber Quality and Cotton Leaf Curl Virus in Gossypium hirsutum L. Accessions.

Author(s): Jehanzeb Farooq | Muhammad Riaz | Frasat Saeed

Date: 2015-05-28 | Type: Journal Article

Genetic diversity in upland cotton for cotton leaf curl virus disease, earliness and fiber quality

Author(s): Jehanzeb Farooq | Abid Mahmood | Tassawar Hussain | Muhammad Riaz | Saghir Ahmad | Frasat Saeed

Date: 2014-12-30 | Type: Journal Article

Recombination Among Begomoviruses on Malvaceous PlantsLeads to the Evolution of Okra Enation Leaf Curl Virus in Pakistan

Author(s): Imran Amin | Khalid Pervaiz Akhtar | Shahid Mansoor | Saad Serfraz

Date: 2014-12-29 | Type: Journal Article

Nutrients Can Enhance the Abundance and Expression of Alkane Hydroxylase CYP153 Gene in the Rhizosphere of Ryegrass Planted in Hydrocarbon-Polluted Soil

Author(s): Muhammad Afzal | Imran Amin | Samina Iqbal | Qaiser M. Khan | Muhammad Arslan

Date: 2014-10-31 | Type: Journal Article

Assessment of genetic diversity for cotton leaf curl virus (CLCuD), fiber quality and some morphological traits using different statistical procedures in Gossypium hirsutum L

Author(s): Frasat Saeed | Jehanzeb Farooq | Abid Mahmood | Muhammad Riaz | Tassawar Hussain | Abdul Majid

Date: 2014-09-30 | Type: Journal Article

Cotton Germplasm of Pakistan

Author(s): Abid Mahmood | Mahboob ur Rehman

Date: 2014-07-02 | Type: Book Chapter

Effects of genetic changes to the begomovirus/betasatellite complex causing cotton leaf curl disease in South Asia post-resistance breaking

Author(s): Rob W. Briddon | Fazal Akbar | Zafar Iqbal | Luqman Amrao | Luqman Amrao | Imran Amin | Muhammad Saeed | Shahid Mansoor

Date: 2014-06-24 | Type: Journal Article

Regional changes in the sequence of cotton leaf curl multan betasatellite

Author(s): Sohail Akhtar | Muhammad Nouman Tahir | Ghulam Rasool | Shaista Javaid | Qaiser M. Khan | Imran Amin | Rob W. Briddon | Shahid Mansoor

Date: 2014-05-23 | Type: Journal Article

Cotton leaf curl virus disease a principle cause of decline in cotton productivity in Pakistan (a mini review)

Author(s): Jehanzeb Farooq | Muhammad Riaz | Tassawar Hussain | Muhammad Farooq | Asia Batool | Tariq Hussain | Abid Mahmood

Date: 2014-02-28 | Type: Journal Article

Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus

Author(s): Imran Amin | Rob W. Briddon | Shahid Mansoor | Irfan Ali

Date: 2013-07-12 | Type: Journal Article

Xanthium strumarium: a weed host of components of begomovirus-betasatellite complexes affecting crops

Author(s): Sohail Akhtar | Imran Amin | Rob W. Briddon | Shahid Mansoor | Muhammad Mubin

Date: 2011-10-04 | Type: Journal Article

NEWS & EVENTS