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Molecular markers and phenotypic characterization of adult plant resistance genes Lr 34, Lr 46, Lr 67 and Lr 68 and their association with partial resistance to leaf rust in wheat

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Abstract

Thirty-nine wheat genotypes were studied to estimate their partial resistance levels to leaf rust at Behira governortae during three growing seasons, i.e. 2016/2017, 2017/2018 and 2018/2019. In these genotypes, partial resistance was characterized using final leaf rust severity (FRS %) and area under disease progress curve (AUDPC). Of the tested genotypes, only three wheat varieties; Giza 171, Misr 3 and Sohag 5 showed complete resistance. Further, 28 of the 39 genotypes had partial resistance as they revealed low and/or moderate values of FRS (%) and AUDPC (not exceeding 30% and 300, respectively). The other eight varieties were fast rusting, as they displayed the maximum values of FRS (%) and AUDPC. The four monogenic lines; Lr 34, Lr 46, Lr 67 and Lr 68 were identified in the wheat varieties using linked molecular markers; csLV34, Xgwm259, CFD71 and csGSR. Phenotypic results of the wheat varieties were confirmed by molecular marker analysis.

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Acknowledgements

We thank Drs Osama Boulot, Aly Abd Alhady, Mohamed Anis Naguib and Sobhy Negm (Plant Pathology Research Institute, ARC, Giza, Egypt) for language corrections and valuable suggestions on the revised manuscript.

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Authors and Affiliations

  1. Wheat Diseases Research Department, Plant Pathology Research Institute, ARC, P.O. Box: 12619, Giza, Egypt

    Walid M. El-Orabey

  2. Biotechnologist, Biodiversity and Integrated Gene Management, International Centre for Agricultural Research in the Dry Areas (ICARDA), P.O. Box: 2416, Giza, Egypt

    Aladdin Hamwieh & Shaimaa M. Ahmed

  3. Agricultural Genetic Engineering Research Institute (AGERI), ARC, P.O. Box: 12619, Giza, Egypt

    Shaimaa M. Ahmed

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  1. Walid M. El-Orabey
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  2. Aladdin Hamwieh
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Correspondence to Walid M. El-Orabey.

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Corresponding Editor: Manoj Prasad

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El-Orabey, W.M., Hamwieh, A. & Ahmed, S.M. Molecular markers and phenotypic characterization of adult plant resistance genes Lr 34, Lr 46, Lr 67 and Lr 68 and their association with partial resistance to leaf rust in wheat. J Genet 98, 82 (2019). https://doi.org/10.1007/s12041-019-1122-1

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  • DOI: https://doi.org/10.1007/s12041-019-1122-1

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