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Genome-wide association studies revealed novel stripe rust resistance QTL in barley at seedling and adult-plant stages

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Abstract

Barley stripe rust, Puccinia striiformis f. sp. hordei, (PSH) occurs worldwide and is one of the major barley diseases of South Asia and North Africa. The aim of this study was to identify and map rust resistance loci at the seedling and the adult plant stages in 336 barley genotypes assembled at ICARDA as AM-2014. The seedling resistance to PSH was evaluated in the greenhouse individually for six virulent races originated from India. The adult stage resistance to PSH was evaluated in field located at Durgapura, Rajasthan, India. The AM-2014 panel was genotyped with infinium iSelect 9K barley SNP markers and genome-wide association studies were carried out using mixed linear model accounting for population structure (PCA) and kinship (K) as covariates. Eleven barley genotypes were identified resistant to all six PSH races at the seedling stage while these genotypes were also resistant at adult plant stage in the field. The most effective SNP markers (at least one for each QTL) associated with rust resistance was also identified using stepwise regression. A total of 17 and 25 resistance QTL to six races of PSH were identified in two- and six-rowed barley at the seedling stage, respectively. While, six and seven resistance QTL to PSH were detected at the adult stage in two- and six-rowed barley, respectively. The novel PSH resistant QTL detected at the seedling and adult stages are important for barley breeding programs globally.

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Acknowledgements

This research was jointly funded by CRP Dryland Cereals program of ICARDA, USAID linkage program (small grants) for CG centers, and ICARDA-ICAR/India collaboration project. Authors are also thankful to research staffs of ICAR-IIWBR at Shimla and Karnal, and RARI, Durgapura, Rajasthan.

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  1. Sanjaya Gyawali

    Present address: NWREC, Washington State University (WSU), 16650 State Route 536, Mount Vernon, WA, 98273, USA

Authors and Affiliations

  1. Biodiversity and Crop Improvement Program (BCIP), ICARDA, Rabat, Morocco

    Sanjaya Gyawali & Ramesh Pal Singh Verma

  2. HudsonAlpha Institute for Biotechnology, Huntsville, AL, 35758, USA

    Sujan Mamidi

  3. USDA-ARS Cereal Crops Research Unit, 1605 Albrecht Blvd N, Fargo, ND, 58102-2765, USA

    Shiaoman Chao

  4. Indian Institute of Wheat and Barley Research (IIWBR), Indian Council of Agricultural Research (ICAR), New Delhi, India

    Subhash C. Bhardwaj, Rajan Selvakumar & Om P. Gangwar

  5. Rajasthan Agricultural Research Institute (RARI), Durgapura, Jaipur, India

    Pradeep S. Shekhawat

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Contributions

SG and RPSV conceived and coordinated the study; SC genotyped AM-2014 panel; SCB, RS, OG, PS, and RA collected phenotypic data on rust resistance; SM and SG analyzed data; SG and SM drafted the manuscript; all co-authors contributed equally reviewing the manuscript.

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Gyawali, S., Mamidi, S., Chao, S. et al. Genome-wide association studies revealed novel stripe rust resistance QTL in barley at seedling and adult-plant stages. Euphytica 217, 3 (2021). https://doi.org/10.1007/s10681-020-02728-1

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