biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 58:681-688, 2014 | DOI: 10.1007/s10535-014-0436-3

The genetic basis of durum wheat germination and seedling growth under osmotic stress

M. Nagel1,*, S. Navakode1, V. Scheibal1, M. Baum2, M. Nachit2, M. S. Röder1, A. Börner1
1 Leibniz Institute of Plant Genetics and Crop Plant Research (IPK Gatersleben), Stadt Seeland, Germany
2 International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria

Durum wheat (Triticum turgidum L. var. durum) is mainly produced under rainfed but often sub-optimal moisture conditions in the Mediterranean basin. A set of 114 durum wheat recombinant inbred lines (RILs) developed from the cross of cultivars Omrabi5 × Belikh2 were tested for the ability to tolerate moisture deficiency at the germination and early seedling growth stage. The stress was imposed by exposing the germinating grain to 12 % polyethylene glycol. It induced a measurable reduction in root length, shoot length, and the percentage of normal seedlings. The germination and seedling growth of Belikh2 were more strongly inhibited than those of Omrabi5, and both parents were outperformed by > 50 % of the RILs. A quantitative trait locus (QTL) analysis was carried out by first assembling a linkage map from 265 informative microsatellites. Composite interval mapping revealed nine QTL spread over seven chromosomes. Five of these were associated with coleoptile length, and one of the five explained nearly 29 % of the relevant phenotypic variance. The coleoptile length was significantly correlated with the seedling growth, plant height, and thousand kernel mass derived from field-grown plants of the same RIL population.

Keywords: drought stress; polyethylene glycol; QTL; recombinant inbred lines; seed size; seed vigour; Triticum durum
Subjects: germination; osmotic stress; drought; polyethylene glycol; QTL; recombinant inbred lines; growth; linkage mapping; wheat

Received: April 2, 2013; Revised: April 29, 2014; Accepted: April 30, 2014; Published: December 1, 2014  Show citation

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Nagel, M., Navakode, S., Scheibal, V., Baum, M., Nachit, M., Röder, M.S., & Börner, A. (2014). The genetic basis of durum wheat germination and seedling growth under osmotic stress. Biologia plantarum, 58(4), 681-688. doi: 10.1007/s10535-014-0436-3
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