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Identification and mapping of the leaf stripe resistance gene Rdg1a in Hordeum spontaneum

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Abstract

Leaf stripe of barley, caused by Pyrenophora graminea, is an important seed-borne disease in organically grown as well as in conventionally grown Nordic and Mediterranean barley districts. Two barley segregating populations represented by 103 recombinant inbred lines (RILs) of the cross L94 (susceptible) × Vada (resistant) and 194 RILs of the cross Arta (susceptible) × Hordeum spontaneum 41-1 (resistant) were analysed with two highly virulent leaf stripe isolates, Dg2 and Dg5, to identify loci for P. graminea resistance. A major gene with its positive allele contributed by Vada and H. spontaneum 41-1 was detected in both populations and for both pathogen isolates on chromosome 2HL explaining 44.1 and 91.8% R 2, respectively for Dg2 and Dg5 in L94 × Vada and 97.8 and 96.1% R 2, respectively for Dg2 and Dg5 in Arta × H. spontaneum 41-1. Common markers in the gene region of the two populations enabled map comparison and highlighted an overlapping for the region of the resistance locus. Since the map position of the resistance locus identified in this report is the same as that for the leaf stripe resistance gene Rdg1a, mapped earlier in Alf and derived from the ‘botanical’ barley line H. laevigatum, we propose that leaf stripe resistance in Vada and H. spontaneum 41-1 is governed by the same gene, namely by Rdg1a, and that Rdg1a resistance could be traced back to H. spontaneum, the progenitor of cultivated barley. PCR-based molecular markers that can be used for marker-assisted selection (MAS) of Rdg1a were identified. An Rdg1a syntenic interval with the rice chromosome arm 4L was identified on the basis of rice orthologs of EST-based barley markers. Analysis of the rice genes annotated into the syntenic interval did not reveal sequences strictly belonging to the major class (nucleotide-binding site plus leucine-rich repeat) of the resistance genes. Nonetheless, four genes coding for domains that are present in the major disease-resistance genes, namely receptor-like protein kinase and ATP/GTP-binding proteins, were identified together with a homolog of the barley powdery mildew resistance gene mlo. Three (out of five) homologs of these genes were mapped in the Rdg1a region in barley and the mlo homolog map position was tightly associated with the LOD score peak in both populations.

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Acknowledgments

We acknowledge Thierry C. Marcel and Rients E. Niks (Wageningen University, The Netherlands) for providing information about marker segregation in the L × V map. We also thank Alberto Gianinetti (CRA-GPG, Genomic Research Center, Italy) for statistical analysis of the data and Nicola Pecchioni (Università di Modena e Reggio Emilia, Italy) for L × V phenotypic data and critical reading of the manuscript. This work was supported by the Italian national projects ‘Proteine e geni per la protezione delle piante’ (‘PROTEO-STRESS’) and ‘Nuove tecnologie molecolari per l’analisi del genoma di organismi di interesse agrario’ (‘AGRO-NANOTECH’) funded by MiPAF.

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Authors and Affiliations

  1. CRA-GPG Genomic Research Centre, Via S. Protaso 302, 29017, Fiorenzuola d’Arda (PC), Italy

    Chiara Biselli, Simona Urso, Letizia Bernardo, Alessandro Tondelli, Gianni Tacconi, Valentina Martino & Giampiero Valè

  2. Biodiversity and Integrated Gene Management Programme, The International Center for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Aleppo, Syria

    Stefania Grando

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  1. Chiara Biselli
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  2. Simona Urso
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  3. Letizia Bernardo
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  4. Alessandro Tondelli
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  5. Gianni Tacconi
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  6. Valentina Martino
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  7. Stefania Grando
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  8. Giampiero Valè
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Correspondence to Giampiero Valè.

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Communicated by B. Keller.

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122_2009_1248_MOESM1_ESM.jpg

ESM Fig S1 CAPS and dCAPS markers linked to the Rdg1a locus derived from rice EST orthologs. Ethidium bromide stained gels were used to resolve restriction enzyme digested fragments amplified from L94 (L), Vada (V), Arta (A), H. spontaneum 41-1 (H) and ten RILs lines to demonstrate allele segregation. DNA fragment sizes (bp) are indicated. (JPG 178 kb)

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Biselli, C., Urso, S., Bernardo, L. et al. Identification and mapping of the leaf stripe resistance gene Rdg1a in Hordeum spontaneum . Theor Appl Genet 120, 1207–1218 (2010). https://doi.org/10.1007/s00122-009-1248-2

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  • DOI: https://doi.org/10.1007/s00122-009-1248-2

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